Determination of ergosterol in organic dust by gas chromatography-mass spectrometry
Axelsson, B., Saraf, A. and Larsson, L.
1995
Journal of Chromatography B: Biomedical Sciences and Applications, 666(1), 77-84
Axelsson, B., Saraf, A. and Larsson, L., (1995), "Determination of ergosterol in organic dust by gas chromatography-mass spectrometry", Journal of Chromatography B: Biomedical Sciences and Applications, 666(1), 77-84.
| Abstract: |
| A gas chromatographic-mass spectrometric method was developed for the determination of ergosterol in organic dust. Samples were hydrolyzed under alkaline conditions, and the hydrolysate was extracted, purified on a silica-gel column, and subjected to derivatization. The limit of detection of the trimethylsilyl ether derivative of ergosterol was approximately 10 pg and that of the tert.-butyldimethylsilyl ether derivative was approximately 20 pg (injected amounts). House dust contained 6-45 g ergosterol/g and iar from a pig barn contained 0.2-0.3 ng ergosterol/ liter. The proposed method can be used as a complement or alternative to microscopy and culturing for measuring fungal biomass in air-borne organic dust. |
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