Comparison of the rodac imprint method to selective enrichment broth for recovery of vancomycin-resistant enterococci and drug-resistant enterobacteriaceae from environmental surfaces
Hacek, D. M., Trick, W. E., Collins, S. M., Noskin, G. A. and Peterson, L. R.
2000 Journal of Clinical Microbiology, 38(12): 4646-4648
Hacek, D. M., Trick, W. E., Collins, S. M., Noskin, G. A. and Peterson, L. R., (2000), "Comparison of the rodac imprint method to selective enrichment broth for recovery of vancomycin-resistant enterococci and drug-resistant enterobacteriaceae from environmental surfaces", Journal of Clinical Microbiology, 38(12): 4646-4648.
Abstract:
We compared the Rodac imprint technique to selective enrichment broth for detecting vancomycin-resistant enterococci (VRE) and multidrug-resistant Enterobacteriaceae (MDRE) on surfaces. Rodac plates contained tryptic soy agar with 5% sheep blood, vancomycin (6 ¦Ìg/ml), ceftazidime (2 ¦Ìg/ml), amphotericin B (2 ¦Ìg/ml), and clindamycin (1 ¦Ìg/ml). Two types of broth were used: brain heart infusion (BHI) and BHI plus vancomycin (6 ¦Ìg/ml) and ceftazidime (2 ¦Ìg/ml) (BHIVC). Of the 46 surfaces cultured for VRE, 12 (26%) were positive. Of the 12 VRE-positive surfaces, 11 (92%) grew from Rodac, 8 (67%) grew from BHIVC, and 7 (58%) grew from BHI. A larger study is needed for MDRE, as only 4 of 43 surfaces were MDRE positive. The Rodac imprint technique successfully recovered VRE from environmental surfaces.
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