Effect of aerosolization, growth phase and residence time in spray and collection fluids on the culturability of cells and spores
Griffiths, W. D., Stewart, I. W., Reading, A. R., Futter, S. J. and Number, P. V.
1998 Journal of Aerosol Science, 27, 5, p 803-820, Pediatrics Volume 101, Number 4
Griffiths, W. D., Stewart, I. W., Reading, A. R., Futter, S. J. and Number, P. V., (1998), "Effect of aerosolization, growth phase and residence time in spray and collection fluids on the culturability of cells and spores", Journal of Aerosol Science, 27, 5, p 803-820, Pediatrics Volume 101, Number 4.
Abstract: |
Efforts are being made to establish a UK-based measurement infrastructure for aerosol and bioaerosols to determine the appropriateness and practicality of validating and certifying microbiological reference materials for bioaerosol standards. One of the prime uses of such bioaerosol standards is the characterization of samplers and assay methods. The effects of aerosolization parameters and residence time in the spray and collection liquids on the bioaerosol culturability are examined. Results indicate that traditional culturing techniques tend to underestimate cell/spore numbers in the collected bioaerosols of the microorganisms used. Total count techniques are therefore recommended. |
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