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Related Concept
  • fungi: assessment, sampling and analysis
  • fungi: MVOC - microbial volatile organic compound
  • VOC: measurement and identification
  • measurement: GC-MS
  • measurement: SPME



    • Related References
  • Lord, H. L. and Pawliszyn, J., (0), Recent advances in solid phase microextraction and membrane extraction with a sorbent interface
  • Nilssona, T., Larsenb, T. O., Montanarellac, L. and Madsena, J. ?., (1996), Application of head-space solid-phase microextraction for the analysis of volatile metabolites emitted by Penicillium species
  • Pawliszyn, J., (2001), Solid phase microextraction



    • Related Articles


  • PCR --polymerase chain reaction



    		•
    		Essay:

    SPME - Solid Phase Microextraction

    "This is a newly developed technique that is undergoing extensive research, it has been gaining in popularity over the last few years due to: Speed ¨C Equilibration can be reached in only 2-30 minutes ¨C ideal for quick screening. Sensitivity ¨C Parts per trillion detection limits have been attained, with an ion-trap detector. The method which was invented in the early 1990's by Prof. Janusz Pawliszyn from the University of Waterloo in Ontario, Canada, uses a small segment of fused silica fibre coated with an appropriate material such as polydimethylsiloxane, this is then held in a syringe-like device. The extraction of analytes from various matrices is then undertaken and the introduction of these into a chromatographic system for analyses is the final step. No solvents are needed in this process, and analyte extraction and pre-concentration are combined in one single step. SPME fibers is a 1 or sometimes 2cm long fused silica fibre coated with a polymeric phase." -- http://www.chemsoc.org/exemplarchem/entries/2002/Garner/

    "Solid-phase microextraction is a sample preparation and sample introduction method in which analytes partition from a sample into a polymer, coated on a fused silica rod of typically 1 cm length by 100 ¦Ìm diameter. The fibre is fastened into the end of a fine stainless steel tube contained in a syringe-like device, and protected by an outer stainless steel needle. The device's plunger is depressed to expose the fibre to the sample matrix, retracted at the end of the sampling time, and then depressed again to expose the fibre to a desorption interface for analysis, typically by GC or HPLC."--http://www.science.uwaterloo.ca/chemistry/pawliszyn/Research/MESI/Recentadvances.htm

    Chen and Pawliszyn first introduced the technology of coupling standard SPME fibre sampling to HPLC analysis in 1995 6. (J. Chen and J. Pawliszyn, Anal. Chem. 67(15), 2530-2533 (1995)).

    SPME is an extraction technology that combines sampling and sample preparation. Since it conception, SPME has been widely used for research applications in pharmaceutical, food, aroma, forensic, environmental and physicochemical properties. Three books on SPME summarize the theory, applications and many practical considerations (1. J. Pawliszyn , Solid Phase Microextraction- Theory and Practice. , Wiley-VCH, New York, USA (1997). 2. J. Pawliszyn (Editor), Applications of Solid Phase Microextraction, The Royal Society of Chemistry, Hertfordshire, UK, 1999. 3. S.A. Scheppers-Wiercinski (Editor), Solid Phase Microextraction: A Practical Guide, Marcel Dekke, New York, USA, 1999) -- Koziel and Novak, 2002, Sampling and sample-preparation strategies based on solid-phase microextraction for analysis of indoor air provided an excellent review

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    tidbits:

    Research publications on SPME at This link was checked on Dec. 2006PubMed



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