Determination of ergosterol on mouldy building materials using isotope dilution and gas chromatography--tandem mass spectrometry
Nielsen, K. F. and Madsenc, J.?.
2000 Journal of Chromatography A, 898(2): 227-234
Nielsen, K. F. and Madsenc, J.?., (2000), "Determination of ergosterol on mouldy building materials using isotope dilution and gas chromatography--tandem mass spectrometry", Journal of Chromatography A, 898(2): 227-234.
Abstract:
Ergosterol content of building materials was quantified using gas chromatography-tandem mass spectrometry (GC-MS-MS) in an ion trap with external ionisation. Hydrolysing the samples by classic extraction at 85ˇăC for 90 min in vials was faster, more precise and safer than microwave assisted extraction. [4-2H2]ergosterol was synthesised and used as internal standard, giving method standard deviation of 5-10% from 10 to 30 ng to 10-15 g ergosterol in the sample. The use of GC-MS-MS meant that no solid-phase extraction clean-up was needed, so one person could easily prepare 40-80 samples per day.
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