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Production of mycotoxins on artificially inoculated building materials

Nielsen, K. F., Thrane, U., Larsen, T. O., Nielsen, P. A. and Gravesen, S.
1998
International Biodeterioration & Biodegradation, 42(1): 9-16

Nielsen, K. F., Thrane, U., Larsen, T. O., Nielsen, P. A. and Gravesen, S., (1998), "Production of mycotoxins on artificially inoculated building materials", International Biodeterioration & Biodegradation, 42(1): 9-16.
Abstract:
Isolates of Stachybotrys chartarum, Aspergillus versicolor and Trichoderma spp. from water damaged Danish buildings were grown on old and new gypsum boards, wood pieces, chipboard, gypsum board with wallpaper and acoustic ceiling tiles. Production of sterigmatocystin and 5-methoxysterigmatocystin by each of 5 isolates of A. versicolor growing on pine wood, wallpaper, gypsum board and chipboard, were detected using HPLC with diode array detection and TLC with AlCl3 staining. After derivatization to the heptafluorobutyrlated ester and using gas chromatography ion trap mass spectrometry, negative ion chemical ionisation, for detection, trichothecenes of the verrucarol type were found in 4 of 5 isolates of S. chartarum growing on old and new gypsum boards. None of 8 Trichoderma isolates produced the trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, fusarenon-X, deoxynivalenol, nivalenol or trichothecenes of the verrucarol or trichodermol type on any of the above mentioned materials.
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Author Information and Other Publications Notes
Nielsen, K. F.
Kristian Fog Nielsen, The Mycology Group, BioCentrum-DTU, Building 221, Technical University of Denmark, DK-2800, Kgs. Lyngby, Denmark
  1. Determination of ergosterol on mouldy building materials using isotope dilution and gas chromatography--tandem mass spectrometry
  2. Determination of fungal spore release from wet building materials
  3. Growth of moulds on building materials under different humidities
  4. Microfungal contamination of damp buildings - examples of risk constructions and risk materials
  5. Mould growth on building materials: Secondary metabolites, mycotoxins and biomarkers
  6. Mycotoxin production by indoor molds
  7. Production of mycotoxins on artificially and naturally infested building materials
  8. The Danish Research Programme: "moulds in Buildings" 1998-2001
  9. The release of fungal spores from water damaged building materials  
Thrane, U.
  1. Determination of fungal spore release from wet building materials
  2. Production of mycotoxins on artificially and naturally infested building materials
  3. The release of fungal spores from water damaged building materials  
Larsen, T. O.
  1. A simple method for collection of volatile metabolites from fungi based on diffusive sampling from Petri dishes  
Nielsen, P. A.
Danish Building and Urban Research, H?rsholm, Denmark
  1. Determination of fungal spore release from wet building materials
  2. Growth of moulds on building materials under different humidities
  3. Microfungal contamination of damp buildings - examples of risk constructions and risk materials
  4. Production of mycotoxins on artificially and naturally infested building materials
  5. Quality control of computational fluid dynamics in indoor environments
  6. The release of fungal spores from water damaged building materials  
Gravesen, S.
Danish Building and Urban Research, H?rsholm, Denmark
  1. Determination of fungal spore release from wet building materials
  2. Microbiology on indoor air'99- what is new and interesting? an overview of selected papers presented in Edinburgh, August, 1999
  3. Microfungal contamination of damp buildings - examples of risk constructions and risk materials
  4. Microfungal contamination of damp buildings: Biological aspects
  5. Production of mycotoxins on artificially and naturally infested building materials
  6. Symptoms associated to work in a water damaged school building
  7. The Danish Research Programme: "moulds in Buildings" 1998-2001
  8. The release of fungal spores from water damaged building materials  


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