Relationship of mould count, ergosterol and ochratoxin A production
Saxena, J., Munimbazi, C., and Bullerman, L. B.
2001
International Journal of Food Microbiology, Volume 71, Issue 1, Pages 29-34
Saxena, J., Munimbazi, C., and Bullerman, L. B., (2001), "Relationship of mould count, ergosterol and ochratoxin A production", International Journal of Food Microbiology, Volume 71, Issue 1, Pages 29-34.
| Abstract: |
| The relationship between viable mould count, ergosterol content and ochratoxin A (OA) formation was studied at different inoculum concentrations of Aspergillus ochraceus NRRL 3174 and Penicillium verrucosum NRRL 3260 grown on sterile long-grain enriched white rice as the substrate. Ergosterol was determined by extraction, saponification and quantification using high performance thin layer chromatography (HPTLC) with UV detection. Ergosterol and ochratoxin A were detected after 3 days of incubation and reached their maximum at 7-10 days of incubation. After that, a decline in the concentrations in both ergosterol and ochratoxin was observed. Ergosterol measurement by HPTLC appeared to be a useful method to detect fungal activity, which corresponded to ochratoxin production. Thus, the ergosterol assay may have a use as an early indicator of potential mycotoxin production. |
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