Relationship of mould count, ergosterol and ochratoxin A production
Saxena, J., Munimbazi, C., and Bullerman, L. B.
2001 International Journal of Food Microbiology, Volume 71, Issue 1, Pages 29-34
Saxena, J., Munimbazi, C., and Bullerman, L. B., (2001), "Relationship of mould count, ergosterol and ochratoxin A production", International Journal of Food Microbiology, Volume 71, Issue 1, Pages 29-34.
Abstract:
The relationship between viable mould count, ergosterol content and ochratoxin A (OA) formation was studied at different inoculum concentrations of Aspergillus ochraceus NRRL 3174 and Penicillium verrucosum NRRL 3260 grown on sterile long-grain enriched white rice as the substrate. Ergosterol was determined by extraction, saponification and quantification using high performance thin layer chromatography (HPTLC) with UV detection. Ergosterol and ochratoxin A were detected after 3 days of incubation and reached their maximum at 7-10 days of incubation. After that, a decline in the concentrations in both ergosterol and ochratoxin was observed. Ergosterol measurement by HPTLC appeared to be a useful method to detect fungal activity, which corresponded to ochratoxin production. Thus, the ergosterol assay may have a use as an early indicator of potential mycotoxin production.
This publication in whole or part might be found online. Check the sources on the related article below. Or use search engines on the web.