Conceptual Reference Database for Building Envelope Research Prev
Next

Surface properties of microbial cells probed at the nanometre scale with atomic force microscopy

Boonaert, C. J. P., Rouxhet, P. G. and Dufrene, Y. F.
2000
Surface and Interface Analysis, 30, 1, p 32-35


Boonaert, C. J. P., Rouxhet, P. G. and Dufrene, Y. F., (2000), "Surface properties of microbial cells probed at the nanometre scale with atomic force microscopy", Surface and Interface Analysis, 30, 1, p 32-35.
Abstract:
Atomic force microscopy (AFM) was used to characterize, under water, the surface morphology and molecular interactions of two types of microbial cells: fungal spores (Phanerochaete chrysosporium) and bacteria (Lactococcus lactis). High-resolution deflection images showed that the spore surface was uniformly covered with patterns of rodlets that were several hundred nanometres in length and had a periodicity of approximately 10 nm. Such surface organization was not detected on the bacterial surface, which showed a sponge-like structure. Force-distance curves revealed very different molecular interactions for the two microorganisms: upon approach, no significant curvature was seen in the contact region for spores, contrary to bacteria, pointing to a difference in cell softness; and upon retraction, no adhesion forces were detected on spores but multiple unbinding events and elongation forces attributed to macromolecular bridging were observed on bacteria. These results show that AFM is a powerful tool for probing the surface properties of native microbial cells on the nanometre scale.

Related Resources:


Related Concepts





CRDBER, at CBS, BCEE, ENCS, Concordia,