| Group |
2020-07 |
Status |
completed |
| Title |
Voltage Level and Syringe Pump Controller for a High Throughput Mammalian Cell Electroporation System |
| Supervisor |
Steve Shih |
| Description |
Electroporation is the technique of applying an electric field to a cell to generate nanopores in the cell surface. Electroporation is a crucial technique in modern genetic engineering pipelines such as CRISPR-Cas9 gene editing. A current challenge in the field is precisely controlling electroporation parameters to optimize for efficiency and cell survival.
A control unit is needed to interface with a microfluidic electroporation device. The controller is required to precisely control at least 2 syringe pumps as well as manage discreet voltage levels for at least 10 unique and programmable on-chip electroporation sites. Additional requirements are for a simple user interface allowing for the user to set the pump rates and voltage levels, as well as surge protection, electroporation site current read out, and on-chip temperature sensors.
|
| Requirement |
Microcontrollers
programming (C/C++, assembly)
stepper motors, DC motors , power MOSFETS for current amplification
PCB design
|
| Tools |
Circuit simulation software
Standard prototyping equipment
|
| Number of Students |
4 |
| Students |
Talal Michael Shuhaber
Muhamad Sarris
Victor Manuel Guerra
Alexia Marie Capitina |
| Comments: |
Email: steve.shih@concordia.ca |
| Links: |
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