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Evaluation of a new personal sampler for enumerating airborne spores

Reponen, T., Grinshpun, S. A., Aizenberg, V. A., Wang, Z., G¨®rny, R. and Willeke, K.
2000
Proceedings of Healthy Buildings 2000, Vol. 1 379


Reponen, T., Grinshpun, S. A., Aizenberg, V. A., Wang, Z., G¨®rny, R. and Willeke, K., (2000), "Evaluation of a new personal sampler for enumerating airborne spores", Proceedings of Healthy Buildings 2000, Vol. 1 379.
ABSTRACT

The performance of a new personal sampler, the Button Aerosol Sampler, was tested under controlled laboratory conditions for total and viable enumeration of airborne microorganisms. Test particles included inert PSL particles, fungal and bacterial spores, and bacterial vegetative cells. The collection efficiency was close to 100% for the entire particle size range studied (0.44 - 5.10 ¦Ìm). Penicillium melinii and Aspergillus versicolor demonstrated consistent culturability when sampling for 30 minutes to 8 hours, while less than 20% of Bacillus subtilis spores were culturable after 4-hour sampling. Pseudomonas fluorescens and Serratia marcescens vegetative cells were culturable only at RH=85% if sampled 10 minutes or less. The Button Aerosol Sampler was found to be suitable for the total enumeration of airborne microorganisms and for the viable enumeration of resistant spores. KEYWORDS: bacteria, fungi, bioaerosol, measurement technique, performance


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Author Information and Other Publications Notes
Reponen, T.
Dr.Tiina Reponen, Research Associate Professor Department of Environmental Health University of Cincinnati
     
Grinshpun, S. A.
  1. Aerodynamic versus physical size of spores: measurement and implication for respiratory deposition
  2. Aerosol characteristics of airborne actinomycetes and fungi
  3. Bioaerosol collection by a new electrostatic precipitator
  4. Characteristics of airborne actinomycete spores
  5. Collection of airborne microorganisms by a new electrostatic precipitator
  6. Collection of airborne spores by circular single-stage impactors with small jet-to-plate distance
  7. Collection of bioaerosol particles by impaction: effect of fungal spore agglomeration and bounce
  8. Collection of fungal spores on air filters and spore reentrainment from filters into air
  9. Development and evaluation of aerosol generators for biological materials
  10. Effect of impact stress on microbial recovery on an agar surface
  11. Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores
  12. Field Testing of New Aerosol Sampling Method With a Porous Curved Surface as Inlet
  13. Fungal fragments as indoor air biocontaminants
  14. Fungal spore source strength tester: laboratory evaluation of a new concept
  15. Improved aerosol collection by combined impaction and centrifugal motion
  16. Inlet sampling efficiency of bioaerosol samplers
  17. Long-term sampling of airbome bacteria and fungi into a non-evaporating liquid
  18. Performance characteristics of the button personal inhalable aerosol sampler
  19. Performance of Air-O-Cell, Burkard, and Button samplers for total enumeration of airborne spores
  20. Release of lead-containing particles from a wall enclosure
  21. Release of Streptomyces albus propagules from contaminated surfaces
  22. Techniques for dispersion of microorganisms into air  
Aizenberg, V. A.
  1. Performance characteristics of the button personal inhalable aerosol sampler
  2. Performance of Air-O-Cell, Burkard, and Button samplers for total enumeration of airborne spores  
Wang, Z.
     
G¨®rny, R.
  1. Release of Streptomyces albus propagules from contaminated surfaces
  2. Size distribution of bacterial and fungal bioaerosols in indoor air  
Willeke, K.
  1. Aerosol characteristics of airborne actinomycetes and fungi
  2. Bioaerosol collection by a new electrostatic precipitator
  3. Characteristics of airborne actinomycete spores
  4. Collection of airborne microorganisms by a new electrostatic precipitator
  5. Collection of bioaerosol particles by impaction: effect of fungal spore agglomeration and bounce
  6. Collection of fungal spores on air filters and spore reentrainment from filters into air
  7. Development and evaluation of aerosol generators for biological materials
  8. Effect of impact stress on microbial recovery on an agar surface
  9. Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores
  10. Fungal fragments as indoor air biocontaminants
  11. Improved aerosol collection by combined impaction and centrifugal motion
  12. Inlet sampling efficiency of bioaerosol samplers
  13. Long-term sampling of airbome bacteria and fungi into a non-evaporating liquid
  14. Performance characteristics of the button personal inhalable aerosol sampler
  15. Performance of Air-O-Cell, Burkard, and Button samplers for total enumeration of airborne spores
  16. Performance of bioaerosol samplers: collection characteristics and sampler design considerations
  17. Release of lead-containing particles from a wall enclosure
  18. Release of Streptomyces albus propagules from contaminated surfaces
  19. Source strength of fungal spore aerosolization from moldy building material
  20. Techniques for dispersion of microorganisms into air  



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