Conceptual Reference Database for Building Envelope Research Prev
Next

Long-term sampling of airbome bacteria and fungi into a non-evaporating liquid

Lin, X., Reponen, T. A., Willeke, K., Grinshpun, S. A., Foarde, K. K. and Ensor, D. S.
1999
Atmospheric Environment, 33, 4291-4298

Lin, X., Reponen, T. A., Willeke, K., Grinshpun, S. A., Foarde, K. K. and Ensor, D. S., (1999), "Long-term sampling of airbome bacteria and fungi into a non-evaporating liquid", Atmospheric Environment, 33, 4291-4298.
Abstract:
Conventional sampling of bioaerosols into liquid impingers can only be performed with water or another low-viscosity liquid as the collection medium. Since these liquids evaporate quickly, sampling is generally limited to short-time periods of 15-30 min. In this study, our recently developed "BioSampler", has been used with a non-evaporating, higher viscosity liquid that does not kill nor grow microorganisms, and thus has been used to sample airborne bacteria and fungi for several hours. In side-by-side comparisons with the conventional AGI-30 impinger, sampling indoor air environments over short-time periods, the BioSampler yielded equivalent or higher culturable counts for bacteria and fungi than the AGI-30 when both samplers were operated for 30 min with 20 ml of phosphate buffer as the collection medium. The bio-efficiency of the AGI-30 decreased rapidly with sampling time until the liquid had evaporated after about h. When the BioSampler was operated for 4 h with non-evaporating heavy white mineral oil, the collection efficiency decreased only moderately due to minimal reaerosolization of collected particles and gentle collection of the viable microorganisms. Samples obtained through long-term sampling with the BioSampler may detect culturable bacteria and fungi at lower ambient concentration levels than is possible through conventional liquid impingement sampling over short-time periods.


click for the This link has not been checked.BioSampler
Related Resources:

This publication in whole or part may be found online at: This link was broken when checked on Dec. 2006here.
Related Concepts

Author Information and Other Publications Notes
Lin, X.
  1. Improved aerosol collection by combined impaction and centrifugal motion  
Reponen, T. A.
     
Willeke, K.
  1. Aerosol characteristics of airborne actinomycetes and fungi
  2. Bioaerosol collection by a new electrostatic precipitator
  3. Characteristics of airborne actinomycete spores
  4. Collection of airborne microorganisms by a new electrostatic precipitator
  5. Collection of bioaerosol particles by impaction: effect of fungal spore agglomeration and bounce
  6. Collection of fungal spores on air filters and spore reentrainment from filters into air
  7. Development and evaluation of aerosol generators for biological materials
  8. Effect of impact stress on microbial recovery on an agar surface
  9. Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores
  10. Evaluation of a new personal sampler for enumerating airborne spores
  11. Fungal fragments as indoor air biocontaminants
  12. Improved aerosol collection by combined impaction and centrifugal motion
  13. Inlet sampling efficiency of bioaerosol samplers
  14. Performance characteristics of the button personal inhalable aerosol sampler
  15. Performance of Air-O-Cell, Burkard, and Button samplers for total enumeration of airborne spores
  16. Performance of bioaerosol samplers: collection characteristics and sampler design considerations
  17. Release of lead-containing particles from a wall enclosure
  18. Release of Streptomyces albus propagules from contaminated surfaces
  19. Source strength of fungal spore aerosolization from moldy building material
  20. Techniques for dispersion of microorganisms into air  
Grinshpun, S. A.
  1. Aerodynamic versus physical size of spores: measurement and implication for respiratory deposition
  2. Aerosol characteristics of airborne actinomycetes and fungi
  3. Bioaerosol collection by a new electrostatic precipitator
  4. Characteristics of airborne actinomycete spores
  5. Collection of airborne microorganisms by a new electrostatic precipitator
  6. Collection of airborne spores by circular single-stage impactors with small jet-to-plate distance
  7. Collection of bioaerosol particles by impaction: effect of fungal spore agglomeration and bounce
  8. Collection of fungal spores on air filters and spore reentrainment from filters into air
  9. Development and evaluation of aerosol generators for biological materials
  10. Effect of impact stress on microbial recovery on an agar surface
  11. Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores
  12. Evaluation of a new personal sampler for enumerating airborne spores
  13. Field Testing of New Aerosol Sampling Method With a Porous Curved Surface as Inlet
  14. Fungal fragments as indoor air biocontaminants
  15. Fungal spore source strength tester: laboratory evaluation of a new concept
  16. Improved aerosol collection by combined impaction and centrifugal motion
  17. Inlet sampling efficiency of bioaerosol samplers
  18. Performance characteristics of the button personal inhalable aerosol sampler
  19. Performance of Air-O-Cell, Burkard, and Button samplers for total enumeration of airborne spores
  20. Release of lead-containing particles from a wall enclosure
  21. Release of Streptomyces albus propagules from contaminated surfaces
  22. Techniques for dispersion of microorganisms into air  
Foarde, K. K.
     
Ensor, D. S.
Environmental Science and Engineering, Research Triangle institute, Research Triangle Park, NC 27709-2194, USA
  1. A guide to the Standard EN12975
  2. Penetration of particles into buildings and associated physical factors. part I: model development and computer simulations  


CRDBER, at CBS, BCEE, ENCS, Concordia,