Evaluation of a new personal sampler for enumerating airborne spores
Reponen, T., Grinshpun, S. A., Aizenberg, V. A., Wang, Z., G¨®rny, R. and Willeke, K.
2000 Proceedings of Healthy Buildings 2000, Vol. 1 379
Reponen, T., Grinshpun, S. A., Aizenberg, V. A., Wang, Z., G¨®rny, R. and Willeke, K., (2000), "Evaluation of a new personal sampler for enumerating airborne spores", Proceedings of Healthy Buildings 2000, Vol. 1 379.
ABSTRACT
The performance of a new personal sampler, the Button Aerosol Sampler, was tested under controlled laboratory conditions for total and viable enumeration of airborne microorganisms. Test particles included inert PSL particles, fungal and bacterial spores, and bacterial vegetative cells. The collection efficiency was close to 100% for the entire particle size range studied (0.44 - 5.10 ¦Ìm). Penicillium melinii and Aspergillus versicolor demonstrated consistent culturability when sampling for 30 minutes to 8 hours, while less than 20% of Bacillus subtilis spores were culturable after 4-hour sampling. Pseudomonas fluorescens and Serratia marcescens vegetative cells were culturable only at RH=85% if sampled 10 minutes or less. The Button Aerosol Sampler was found to be suitable for the total enumeration of airborne microorganisms and for the viable enumeration of resistant spores. KEYWORDS: bacteria, fungi, bioaerosol, measurement technique, performance |
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